Vacancy expired!
- Identify suitable genetic tools (RNAi and/or CRISPR) to study the cellular phenotypes of target knockdown
- Profile the consequences of cellular target knockdown using techniques including Western Blotting, ELISA, qPCR, flow cytometry, cell viability and proliferation assays, etc.
- Optimize cell-based assays (eg. AlphaLISA, In Cell Western Blotting, etc.) to enable medium- to high throughput readout of cellular target function and inhibition
- Execute cell-based assays to screen for activity of small molecule inhibitors and to characterize their mechanism of action
- Independently design cell and molecular biology experiments, acquire, analyze and interpret data, and communicate findings to project team
- Document experiments in lab notebook, and write up experimental protocols
- Present at and actively participate in project team meetings
- Work collaboratively as a member of a multidisciplinary project team
- BS/MS degree in Cell or Molecular Biology, Bioengineering or another relevant life science field
- A minimum of 2+ years of relevant research experience in a biotechnology or pharmaceutical industry setting
- Background in cancer biology and profiling of small molecules preferred
- Extensive hands-on experience culturing adherent and suspension cancer cell lines
- Demonstrated expertise developing and executing cell-based assays such as AlphaLISA, In Cell Western Blotting, and cell proliferation assays; accustomed to the use of bioluminescence and fluorescence plate readers and skilled in the analysis of resulting data
- Proficiency in Western Blotting, ELISA, and qPCR
- Familiarity with DNA cloning, RNAi and/or CRISPR tools, transfection, lentiviral transduction, and flow cytometry desired
- Highly organized with great attention to detail and strong record keeping skills
- Enthusiastic and motivated with a positive can-do attitude and demonstrated problem-solving competencies
- Excellent verbal and written communication skills
- Ability to work in a team setting
- Health insurance
- Casual Dress code
- 401k match
Vacancy expired!